CHROMABOND® QuEChERS extraction buffer mixes / clean-up mixes

QuEChERS method and pre-mixes
Within a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.
Standard clean-up of food samples
10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO4 and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND® Diamino and 150 mg MgSO4 and shaken again. After centrifugation the supernatant is injected into the GC/MS.

Extraction mixtures for sample preparation for determination of pesticides in food samples.

MACHEREY-NAGEL offers a number of individually weighed and pre-mixed buffers and extraction mixtures, specially composed for different sample matrices.
Mix I and Mix II are extraction mixtures, Mix III to Mix VI are clean-up mixtures.

The food samples will be extracted with either Mix I or Mix II.
Afterwards they will be purified with one of the following mixtures:
  • Mix III (samples with low fat content; e.g. apples, strawberries),
  • Mix IV (moderate content of chlorophyll and carotinoids; e. g. carrots, lettuce),
  • Mix V (high content of chlorophyll and carotinoids; e. g. bell peppers, spinach),
  • Mix VI (high fat content; e. g. avocados).

Every mix is prepacked in a 15 ml centrifuge tube. Pack of 50 pieces.

Composition: 0.15g CHROMABOND® Diaminowith 0.9g MgSO4and 45mg Carbon
Description: Mix V Diamino/Carbon Clean-up Mix
Dual Use: 0
Manufacturer Article Number: 730975
Volume ml: 15

Similar products

Accessories for CHROMABOND®
* one filter element is already inserted in the column.
From CHF 229.80*

CHROMABOND® Florisil®
Magnesium silicate for SPEMatrix magnesium silicate (MgO to SiOH 15:85), high purity, particle size 150 to 250µm.Recommended application: organic tin compounds, aliphatic carboxylic acids, PCB, PAH.*Glass columns
From CHF 150.80*

CHROMABOND® Na2SO4/Florisil®
Combination phase for SPE of hydrocarbons from water acc. to DIN H53 / ISO DIS 9377-4Special combination phase of sodium sulphate and Florisil®.Recommended application: hydrocarbons from drinking, surface and waste waters.*Glass columns
From CHF 263.10*

CHROMABOND® C18 Hydra
Octadecyl modified silica phase for SPE of polar analytesBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8, special octadecyl phase for polar analytes, not endcapped, carbon content 15%.Recommended applications: more polar compounds like pesticides and their polar degradation products, phenols, phenoxycarboxylic acids, nitroaromatics, pharmaceuticals.
From CHF 148.70*

CHROMABOND® PS-OH- / PS-H+
The innovative cation exchanger offers:mixed-mode SPE phase with distinct cation exchange and RP properties excellent enrichment of basic analytesmodern support polymer with optimised pore structure and large specific surfacegood reproducibility, reliable and cost-efficient analysesmore aggressive washing procedures for matrix removal possiblecleaner samples and protection of analytical HPLC and GC instrumentationquantificationof analytes from heavily contaminated sampleslower limits of detection also for critical matrices... the perfect fit for basic analytes in comination with CHROMABOND® HR-XSPE phases for polymer-based RP and ion chromatographyBase material: high-purity polystyrene-divinylbenzene copolymers (PS/DVB) pore size 100 Å, paricle size 100µm. Verly low degree of swelling, thus very well suited for chromatography. Different modifications for different applications from elimination of nonpolar compounds up to removal of specific polar comonents.Recommended application:Removal of interfering compounds improves chromatographic separtions, if the interfering components overlap with the analyte in the chromatogramimproves lifetime of the chromatographic column, since interfering components can irreversibly block the column packingremoval or concentration of anions from water increasing the pH value in acidic samples removal or concentration of cations from waterPS-OH- strong PS/DVB anion exanger, OH- form capacity 0,6 meq/gdecreasing the pH value in basic samplesPS-H+ strong PS/DVB cation exchanger, H+ form, capacity 2,9 meg/g
From CHF 165.40*

Empty columns CHROMABOND®, PP
Empty CHROMABOND® column (PP) for SPE, one PE-filter element is already inserted in the column.
From CHF 101.90*

CHROMABOND® C18 ec
Octadecyl modified silica phase for SPE, endcappedBase material silica, pore size 60 Å, particle size 45 µm for C18 ec, 100 µm for C18 ec f (for fast flow), specific surface 500 m²/g, pH stability 2 to 8. Octadecyl phases, endcapped, carbon content 14 % very non-polar, hydrophobic interactions with a wide variety of organic compounds. Advantageous for clean-up of samples with large structural variations (polarity differences).Recommended applications: non-polar compounds, aflatoxins, amphetamines, antibiotics, antiepileptics, barbiturates, caffeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitamins. Very well suited for desalting of samples.BIGpacks: 4.003 446 / 4.003 450 / 4.003 452
From CHF 251.70*

CHROMABOND® SB
Quaternary ammonium modified silica anion exchanger for SPE (SAX)Base material silica, pore size 60 Å, particle size 45 µm, specific surface 500 m²/g, pH stability 2 to 8. Silica modified with quaternary amine. Strongly basic anion exchanger (capacity ~ 0.3 meq/g). Not suited for very strong anions such as sulphonic acids, because these are difficult to elute.Recommended application: organic acids, caffeine, saccharin.BIGpacks: 4.003 487
From CHF 193.40*

CHROMABOND® Alox A / Alox N / Alox B
aluminium oxide, acidic, neutral, basicaluminium oxide, high purity, pore volume 0.90ml/g, particle size 60 to 150µm, specific surface 150m²/g, acidic, pH 4 ± 0.5, recommended application: together with phase SA for PCB and pesticides.Alox A: acidic, pH 4 ± 0.5Alox N: neutral, pH 7 ± 0.5Alox B: basic, pH 9.5 ± 0.5
From CHF 155.00*

CHROMABOND® XTR
Kieselguhr phase for liquid-liquid extractionBase material coarse-grained kieselguhr, large pore size, high pore volume, constantly high batch-to-batch quality, pH working range 1 to 13.Application: liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis.Glass columns availlable on request.
From CHF 120.60*